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ICH Linearity Studies using Automated Standard Preparation and UHPLC

Fraser McLeod (Dionex Softron GmbH)

According to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH), it is necessary to test analytical methods for their linearity as part of a method validation study. The typical process for this is to prepare standards for five different calibration levels, and to make triplicate injections of each of these standards. The concentration range used is typically 70–130 % of the nominal concentration.

Fig. 1 Analysis of 5 soft drinks analytes in less than 5 minutes

Statistically it is recommended that each concentration level is prepared individually. This has the effect of randomising potential sources of error (e.g. a possible incorrect weighing of one of the standards). However, this is typically not done as it is a time consuming process. Instead, many laboratories prepare a stock solution, and then dilute this down to the five different concentration levels. This has the benefit of being the fastest way of preparing standards, but has a major disadvantage: any error in the stock solution will be carried through to the diluted standards.

Tab. 1 Concentrations of soft drinks analytes prepared by the Quantos QB1-L-System

Many chromatography methods determine more than one analyte, and each analyte must be tested for linearity.
This substantially increases the time (and chance of error) for manual preparation and also increases the chance of error for the stock standard/dilution approach. This means that all approaches have drawbacks.

Tab. 2 Correlation coefficients of all analytes

To automate the preparation of the standards, using the new Quantos QB1-L system from Mettler-Toledo is a more suitable method. The system makes it possible to auto­matically dispense and weigh analytes into HPLC vials or volumetric flasks. It can also weigh in the appropriate amount of diluents in order to provide a gravimetric solution. For linearity studies this has many advantages, but the major one is that the time issue is no longer a critical factor and that it is possible to use a statistically correct approach of preparing each standard concentration individually.

In this experiment we tested the linearity of five analytes in a soft drinks analysis. The analytes were acesulfame K, saccharin, caffeine, vanillin and benzoic acid. The Quantos system automatically weighed the correct amount of each analyte, and then the correct amount of diluent (90:10 water: methanol) to provide the concentrations shown in Tab. 1. The time taken to prepare these standards was only 50 minutes. This is a significant reduction in time, ­compared directly to the manual preparation (approx. 3 hour for the stock solution approach, and 4 hours for the manual approach in single steps).

Once the solutions are prepared, the actual analysis follows. In classical HPLC this could take about 30 minutes per analysis. This means that the total run time for the linearity experiment would be 7.5 hours (5 calibration levels x 3 injections per level x 30 minutes). However, with UHPLC it is possible to decrease this run time substantially. Figure 1 shows the chromatographic analysis of all 5 analytes. All peaks are separated in less than 4 minutes, and the total run time is only 5 minutes. This means that all injections can be performed in only 1.25 hours.

Tab. 3 Time required to perform a linearity experiment using the traditional process and the new automated process

This analysis was performed on the Dionex UltiMate® 3000 Basic Automated System – an entry level system that is fully UHPLC compatible. The system supports pressures up to 620 bar and flow rates as high as 10 ml/min, and is ideal for running fast routine analyses. Further speed-up of the analysis would be possible with the UltiMate 3000 Rapid Separation LC System, as this supports pressures as high as 1000 bar.

Once all data has been acquired it is necessary to calculate the results. ICH requires that the following values are reported; correlation coefficient, y-intercept, slope of the regression line, and residual sum of squares. Further, it needs to be checked that the correlation coefficient is within the limits expected of the method (typically >= 0.999). Performing these calculations can be a time consuming task. Some laboratories use Excel spreadsheets in an attempt to speed-up the process, but even this can be time consuming as users typically need
to manually transcribe values into the spread­sheet and another person has to review this transcription.
For this sample analysis, the use of spreadsheets, and the as­sociat­ed review step, would take about 2 hours.
The Chromeleon® Chromatography Data System from Dionex can fully automate this task, and immediately generate all results for all analytes. All that is required is for the user to name the peaks and input their concentration data – a process that takes only 5 minutes. Figure 2 shows the automatically generated report for the analyte “Saccharin”.

By combining both systems an outstanding result for the linearity experiment could be achieved (see Tab. 2).
The R2 value for all analytes is greater than 0.999.

In addition, the automation of the linearity workflow through the combined use of  Quantos QB1-L from Mettler-Toledo together with the UltiMate 3000 system and ­Chromeleon software provides vast potential in realising significant productivity advantages (see Tab. 3).

Fig. 2 Linearity report for Saccharin

More about Dionex

  • Authors

    Dr. Mark Tracy

    Mark Tracy graduated in chemistry from the University of California, Davis, obtaining his Ph.D. there in 1984. He then worked as a chemist in the U.S. Air Force and at the California Animal Health and Food Safety (CAHFS) Laboratory System and Pickering Laboratories, Inc. In 2001, he began w ... more

    Dr. Carsten Paul

    Carsten Paul studied chemistry (focus ­environmental chemistry)  at Friedrich Schiller University (FSU) Jena from 2004 to 2008. After a short spell at the Scripps Institute for Oceano­graphy (La Jolla/CA, USA) he completed his doctorate on a scholarship from the Jena School for Microbial Co ... more

    Dr. Frank Steiner

    Frank Steiner graduated in chemistry before obtaining his doctorate in 1995 from Saarland University in Saarbrücken. This was followed by a postdoc position at the Centre d'Études Nucléaires de Saclay in France, where he worked on elemental analysis and isotope analysis with IC and IC-ICP/M ... more

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