q&more
My watch list
my.chemie.de  
Login  

News

New technology for enzyme design

AK Seibel / Uni Würzburg

The surface of the enzyme levansucrase has been redesigned to produce sugar polymers.

05-Jun-2018: Scientists at the University of Würzburg have chemically modified the enzyme levansucrase using a new method. The enzyme can now produce sugar polymers that are exciting for applications in the food industry and medicine.

Enzymes are tools of nature that accelerate almost all biochemical reactions in living cells as biological catalysts. For this reason, enzymes have been used in the chemical industry for some time now - in detergents and cleaners, toothpastes and shampoos, but also in foods. Enzymes help in the production of paper, textiles, leather, medicines, biofuels and other products.

Enzymes from the tailoring industry

Biochemically, enzymes are proteins that are composed of natural amino acids. They form a three-dimensional structure. Just like a key fits into a lock, each specific molecule fits into an enzyme and the enzyme converts it into a new product.

Technically, it is possible to exchange individual amino acids in an enzyme and thereby change its structure so that it can now process other molecules. In this way, British scientists have created just recently a mutant enzyme that breaks down plastic.

Surface of the levansucrase changed

Chemists from Julius-Maximilians-Universität Würzburg (JMU) in Bavaria, Germany, have now gone a step further in the tailoring of enzymes: "We thought what fascinating possibilities would arise if we could change the surface of enzymes chemically and use the chemical space of molecules," says Jürgen Seibel , Professor of Organic Chemistry at the JMU. "We have developed a reaction that does not occur in nature in this way. It gives us a lot of freedom in reshaping enzyme surfaces. "

As the JMU scientists report in the journal "Chemical Science", they first redesigned the surface of the enzyme levansucrase. Now, the enzyme can convert the table sugar (sucrose) directly into a polymer of fructose building blocks.

"So far, such a synthesis has been possible with levansucrase, but it works much more efficiently with the modified enzyme," explains Seibel. The conversion of the enzyme per second is now significantly higher; moreover, it mainly produces the desired product and no accidental by-products.

Interesting for medicine and food industry

The fructose polymer could be used as a bio-gel for tissue transplantation in medicine or in the food industry – for example as a probiotic supplement in yogurts or baby food. Because like other functional sugars, the polymer could also serve certain intestinal bacteria as food and indirectly exert a health-promoting influence on the intestinal flora of humans.

Original publication:
Maria Elena Ortiz-Soto, Julia Ertl, Jürgen Mut, Juliane Adelmann, Thien Anh Le, Junwen Shan, Jörg Teßmar, Andreas Schlosser, Bernd Engels, Jürgen Seibel; "Product-Oriented Chemical Surface Modification of a Levansucrase (SacB) via an Ene-type Reaction"; Chemical Science; 2018

Facts, background information, dossiers

  • enzymes
  • amino acids
  • medicine
  • sugar polymers

More about Uni Würzburg

  • News

    Doped Photovoltaics

    Organic solar cells are made of cheap and abundant materials, but their efficiency and stability still lag behind those of silicon-based solar cells. A Chinese-German team of scientists has found a way to enhance the electric conductivity of organic solar cells, which increases their perfor ... more

    Cell Division at High Speed

    In malignant tumours, the cells usually proliferate quickly and uncontrollably. A research team from the Biocenter of Julius-Maximilians-Universität (JMU) Würzburg in Bavaria, Germany, has discovered that two important regulators of cell division can interact in this process. If this is the ... more

    Inert Nitrogen Forced to React with Itself

    Direct coupling of two molecules of nitrogen: chemists from Würzburg and Frankfurt have achieved what was thought to be impossible. This new reaction is reported in Science magazine and opens new possibilities for one of the most inert molecules on earth. Constituting over 78 % of the air ... more

  • q&more articles

    High-tech in the beehive

    Healthy honeybee colonies are crucial to maintaining the natural diversity of flowering plants and the global production of plant-derived foodstuffs. As much as 35 % of this production depends on insect-based pollination, in which the honeybee (Apis mellifera) plays a leading role. For fund ... more

  • Authors

    Prof. Dr. Jürgen Tautz

    studied biology, geography and physics at the University of Konstanz before receiving his doctorate from the University on an ecology-related subject. Work in insect, fish and frog bio-acoustics was followed by his foundation of the BEEgroup at the University of Würzburg in 1994, a group th ... more

  • Videos

    High-tech in the beehive

    more

q&more – the networking platform for quality excellence in lab and process

The q&more concept is to increase the visibility of recent research and innovative solutions, and support the exchange of knowledge. In the broad spectrum of subjects covered, the focus is on achieving maximum quality in highly innovative sectors. As a modern knowledge platform, q&more offers market participants one-of-a-kind networking opportunities. Cutting-edge research is presented by authors of international repute. Attractively presented in a high-quality context, and published in German and English, the original articles introduce new concepts and highlight unconventional solution strategies.

> more about q&more

q&more is supported by:

 

Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE