Today, the guidelines of „Good Manufacturing Practice“ (GMP) are the basis for any production and marketing of active pharmaceutical ingredients (APIs) and drugs. Comprehensive quality management systems document and control the production processes and environment and not least the analyti ... more
Industrial chromatography and reverse osmosis optimize amino acid fermentation process
06-Feb-2015: Using creativity and unconventional ideas, researchers and engineers at Evonik have achieved a significant breakthrough in the biotechnological production of amino acids based on fermentation. They integrated the separating methods of chromatography and reverse osmosis and an online analytical method based on spectroscopy—all of which tend to be used in other industry sectors—in a biotechnological process. This created an outcome experts had previously considered nearly impossible. The researchers' objective was to achieve optimal separation of the desired amino acid from a fermentation broth.
"Innovation must be preceded by a good idea and the ability to think outside the box. Our scientists need self-initiative and persuasiveness to turn an idea into an innovation. The innovation that managed to increase the amino acid yield is so successful that it won our internal Innovation Award," said Dr. Ulrich Küsthardt, Chief Innovation Officer of Evonik.
In the fermentative production of amino acids, Evonik exploits the ability of microorganisms to produce complex molecules such as certain amino acids in a vessel at moderate conditions. The downstream purification and treatment process is also a key factor in product quality and yield. The special challenge of the downstream fermentation process is that the composition of the fermentation broth fluctuates considerably. This overstrains many of the proven separating technologies and they are less efficient than they should be.
The skillful combination of the three methods brought the desired success: a continuous chromatography process that separates the amino acids from the fermentation broth with high selectivity, thereby increasing product yields; a reverse osmosis process that removes water from the broth and, combined with the chromatography process, significantly increases the efficiency of the entire process; and a process monitoring system based on near-infrared spectroscopy allows to operate the process in the optimal range.
Evonik Fermas in Slovakia has been using this expanded downstream process successfully for the past two years. It has led to significant improvements in product yields, productivity, production costs, and environmental performance. The new findings have also been transferred to other fermentation processes. But the process experts aren't stopping here: Based on their findings they are currently studying how to reduce the use of thermal energy in the production of amino acids even further.